Freedom Wears a Cap : The Law, Liberty, and Opportunity for British Convict Servants in Virginia, 1718-1788

Great Britain’s passage of the Transportation Act of 1718 was intended to relieve Great Britain of an unwanted criminal element while at the same time providing much needed labor for her North American colonies. This thesis argues that the legislative body of Virginia initially responded by passing legislation intended to limit the dangers presented by the introduction of convict servants into the colony. However, the significant demand for labor in Virginia resulted in the colony receiving a substantial share of those convicts transported to North America. Contemporaries argued that the importation of convict servants led to an increase in crime. This study employs the court records of eighteen counties in Colonial Virginia. The author contends that the evidence reveals that theft was the most common criminal activity of convict servants in Virginia. However, despite claims to the contrary, convict servants did not commit crimes in excess of their percentage of the tithable population. Moreover, over the course of the eighteenth-century justices county justices increasingly relied on providing lighter sentences than that prescribed by the law. This enabled planters to punish the criminal while maintaining control over their labor. The last chapter moves away from the legal system of Virginia and examines the opportunities the American Revolution provided to convict servants

The Orion constellation as an installation - An innovative three dimensional teaching and learning environment

Visualising the three dimensional distribution of stars within a constellation is highly challenging for both students and educators, but when carried out in an interactive collaborative way it can create an ideal environment to explore common misconceptions about size and scale within astronomy. We present how the common table top activities based upon the Orion constellation miss out on this opportunity. Transformed into a walk-through Orion installation that includes the position of our Solar system, it allows the students to fully immerse themselves within the model and experience parallax. It enables participants to explore within the installation many other aspects of astronomy relating to sky culture, stellar evolution, and stellar timescales establishing an innovative learning and teaching environment.Comment: 2 pages, submitted to The Physics Teacher - Colum

Activity of species-specific antibiotics against Crohnʼs disease–associated adherent-invasive Escherichia coli

Background: Crohn's disease (CD) is associated with bacterial dysbiosis that frequently includes colonization by adherent-invasive Escherichia coli (AIEC). AIEC are adept at forming biofilms and are able to invade host cells and stimulate the production of proinflammatory cytokines. The use of traditional antibiotics for the treatment of CD shows limited efficacy. In this study, we investigate the use of species-specific antibiotics termed colicins for treatment of CD-associated AIEC. Methods: Colicin activity was tested against a range of AIEC isolates growing in the planktonic and biofilm mode of growth. Colicins were also tested against AIEC bacteria associated with T84 intestinal epithelial cells and surviving inside RAW264.7 macrophages using adhesion assays and gentamicin protection assay, respectively. Uptake of colicins into eukaryotic cells was visualized using confocal microscopy. The effect of colicin treatment on the production of proinflammatory cytokine tumor necrosis factor alpha by macrophages was assessed by an enzyme-linked immunosorbent assay. Results: Colicins show potent activity against AIEC bacteria growing as biofilms when delivered either as a purified protein or through a colicin-producing bacterial strain. In addition, colicins E1 and E9 are able to kill cell-associated and intracellular AIEC, but do not show toxicity toward macrophage cells or stimulate the production of proinflammatory cytokines. Colicin killing of intracellular bacteria occurs after entry of colicin protein into AIEC-infected macrophage compartments by actin-mediated endocytosis. Conclusions: Our results demonstrate the potential of colicins as highly selective probiotic therapeutics for the eradication of E. coli from the gastrointestinal tract of patients with CD